Phospho-GSK3α-S21 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74159-50, BS74159-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P49840

Host: Rabbit

Reactivity: Human

Applications: WB, IP,

All Applications: WB,1:500 - 1:2000 | IP,1:50 - 1:100

Background: This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Bioworld Molecular Weight: 51kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of 293 and HeLa cells, using Phospho-GSK3α-S21 antibody at 1:1000 dilution. 293 cells were treated by Insulin for 10 minutes after serum-starvation overnight. HeLa cells were treated by Insulin for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA., Western blot analysis of extracts of 293 cells, using Phospho-GSK3α-S21 antibody at 1:1000 dilution or GSK3α antibody. 293 cells were treated by Insulin for 10 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA., Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-GSK3α-S21 pAb. Western blot was performed from the immunoprecipitate using Phospho-GSK3α-S21 pAb at a dilution of 1:1000. 293T cells were treated by Insulin at 37℃ for 10 minutes after serum-starvation overnight.

Alternative Name: GSK3 alpha; GSK3A

Immunogen: A synthetic phosphorylated peptide around S21 of human GSK3α(NP_063937.2).

Conjugate: Unconjugated

Modification: Phosphorylated