Raf1 (Phospho-S43) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS94091-50, BS94091-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P04049(Human) Q99N57(Mouse) P11345(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB:1:1,000-1:2,000

Background: Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk)and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-1 is activated in response to activation of a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. There is accumulating evidence that Ras p21 may play a role in activation of Raf-1 and may play the role of the messenger from membrane tyrosine kinases to Raf-1.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho-Raf1 (S43) on differentLysates using anti-Phospho-Raf1 (S43) antibody at 1/1,000 dilution. Positive control:
Lane 1: NIH/3T3
Lane 2: 293T

Bioworld Molecular Weight: 73 kDa

Specificity: Raf1 (Phospho-S43) polyclonal antibody detects endogenous levels of Raf1 protein only when phosphorylated at S43.

Alternative Name: cRaf, RAF1, c Raf, C-raf, C-Raf proto-oncogene, serine/threonine kinase, CMD1NN, Craf 1 transforming gene, cRaf, Craf1 transforming gene, EC 2.7.11.1, kinase Raf1, Murine sarcoma 3611 oncogene 1, NS5, Oncogene MIL, Oncogene RAF1, OTTHUMP00000160218, OTTHUMP00000207813, OTTHUMP00000209389, Protein kinase raf 1, Proto-oncogene c-RAF, Raf 1, Raf 1 proto oncogene serine/threonine kinase, RAF, Raf proto oncogene serine/threonine protein kinase, RAF proto-oncogene serine/threonine-protein kinase, RAF-1, RAF1, RAF1, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1, TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV, v raf 1 murine leukemia viral oncogene homolog 1, v-raf murine sarcoma viral oncogene homolog 1, v-raf-1 murine leukemia viral oncogene-like protein 1, vraf1 murine leukemia viral oncogene homolog 1,

Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser43 of human Raf1.

Conjugate: Unconjugated

Modification: Phosphorylation