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Modification of Bold's recipe. General purpose freshwater medium used for xenic cultures.
| # | Component | Amount | Stock Solution Concentration | Final Concentration |
|---|---|---|---|---|
| 1 | NaNO3 (Fisher BP360-500) | 10 mL/L | 10 g/400mL dH2O | 2.94 mM |
| 2 | CaCl2•2H2O (Sigma C-3881) |
10 mL/L | 1 g/400mL dH2O | 0.17 mM |
| 3 | MgSO4•7H2O (Sigma 230391) |
10 mL/L | 3 g/400mL dH2O | 0.3 mM |
| 4 | K2HPO4 (Sigma P 3786) |
10 mL/L | 3 g/400mL dH2O | 0.43 mM |
| 5 | KH2PO4 (Sigma P 0662) |
10 mL/L | 7 g/400mL dH2O | 1.29 mM |
| 6 | NaCl (Fisher S271-500) |
10 mL/L | 1 g/400mL dH2O | 0.43 mM |
| 7 | P-IV Metal Solution | 6 mL/L | ||
| 8 | Vitamin B12 | 1 mL/L | ||
| 9 | Biotin Vitamin Solution | 1 mL/L | ||
| 10 | Thiamine Vitamin Solution | 1 mL/L |
Ships within 48 hours · Estimated delivery Jun 19 - Jun 24
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