20% OFF shipping at svevole.cz on orders over $79 + up to 10% OFF products
cz
home > Bold 1NV Medium > Bold 1NV Medium
download picture
Bold 1NV MediumBold 1NV Medium Modification of Bold's recipe. General purpose freshwater medium used for xenic cultures. Directions For 1 L Total To approximately 900 mL of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously. Bring the total volume to 1 L with dH2O. For 1. 5% agar medium add 15 g of agar into the flask; do not mix. Cover and autoclave medium. When cooled add vitamins. For agar medium add vitamins,
Shopping security

Shopping security

Each payment you make on thelockerguy is secured with strict SSL encryption and PCI DSS data protection protocols

Bold 1NV Medium

Modification of Bold's recipe. General purpose freshwater medium used for xenic cultures.

Directions

For 1 L Total

  1. To approximately 900 mL of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously.
  2. Bring the total volume to 1 L with dH2O.
    • For 1.5% agar medium add 15 g of agar into the flask; do not mix.
  3. Cover and autoclave medium.
  4. When cooled add vitamins.
    • For agar medium add vitamins, mix, and dispense before agar solidifies.
  5. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 NaNO3 (Fisher BP360-500) 10 mL/L 10 g/400mL dH2O 2.94 mM
2 CaCl22H2O
(Sigma C-3881)
10 mL/L 1 g/400mL dH2O 0.17 mM
3 MgSO47H2O
(Sigma 230391)
10 mL/L 3 g/400mL dH2O 0.3 mM
4 K2HPO4
(Sigma P 3786)
10 mL/L 3 g/400mL dH2O 0.43 mM
5 KH2PO4
(Sigma P 0662)
10 mL/L 7 g/400mL dH2O 1.29 mM
6 NaCl
(Fisher S271-500)
10 mL/L 1 g/400mL dH2O 0.43 mM
7 P-IV Metal Solution 6 mL/L
8 Vitamin B12 1 mL/L
9 Biotin Vitamin Solution 1 mL/L
10 Thiamine Vitamin Solution 1 mL/L

Bold 1NV Medium

Item no : 47431278156
sold recently : Login >>
US$ 55.00
Pay in 4 interest-free payments of $13.75 Learn more
Min. order: 1piece

Shipping Estimate
USA
  • USA
  • CAN

Ships within 48 hours · Estimated delivery Jun 19 - Jun 24

Enjoy 20% off shipping

US$ 55.00

1-11

US$ 49.50

12-35

US$ 38.50

36-59

US$ 33.00

60+

US$40

Get now

Sign up to your membership to get coupons up to

15%

Get now

Opportunity to enjoy order discount up to 15% off

Please add the products
Shipping Notes
  • Free Standard Shipping on $100+ Orders to the USA.
  • Except Preorder products are shipped in 48 hours.
  • Delivery to the USA:
  1. Standard Shipping : 3-10 business days
  • If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy

Discover Niche Categories That Outsell

Top-Converting Item to Boost Your Average Order

recommand products

Related Searches