HSP27 (phospho-S82) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS4762-50, BS4762-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P04792

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP,

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IP: 1:10~1:50

Background: Hsp27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. Hsp27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. This protein is involved in stress resistance and actin organization. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein. Because of the estrogen-responsive nature of Hsp27, this protein has been studied extensively in human estrogen-responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that Hsp27 may be a useful marker in classifying various hormone sensitive tumors.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: HSP27 (phospho-S82) polyclonal antibody detects endogenous levels of HSP27 protein only when phosphorylated at Ser82.

Bioworld Molecular Weight: ~ 27 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of HSP27 (phospho-S82) pAb at 1:500 dilution
Lane 1: A375 whole cell lysate
Lane 2: SGC7901 whole cell lysate
Lane 3: HEK293T whole cell lysate
Lane 4: HEK293T transfected with pCDNA3.1-Flag-HSP27 whole cell lysate
Lane 5: HEK293T incubated at 42ºC for 1 hour whole cell lysate, Immunohistochemistry (IHC) analyzes of p-HSP27 (S82) pAb in paraffin-embedded human breast carcinoma tissue at 1:100., Immunoprecipitation of L02 whole cell lysate using HSP27 (phospho-S82) pAb (Sepharose Bead Conjugate) #BD0047(Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (Lane 4 and Lane 5). Lane 1 is 20% input. The western blot was probed using HSP27 (phospho-S82) pAb #BS4762. “↑”（supernatant); “↓”（deposition）

Alternative Name: Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; HSPB1; HSP27; HSP28

Immunogen: Synthetic phosphopeptide derived from human HSP27 around the phosphorylation site of Serine 82.

Conjugate: Unconjugated

Modification: Phosphorylation