Recombinant Human NACHRA5/CHRNA5 Protein (His Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH033582-10, PKSH033582-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: NACHRA5; CHRNA5

Target Symptom: Neuronal Acetylcholine Receptor Subunit Alpha-5; CHRNA5; NACHRA5

Research Area: Neuroscience

Target Species: Human

Expression Host: HEK293 Cells

Fusion Tag: C-His

UNIProt ID: P30532

Accession: P30532

Background: Neuronal Acetylcholine Receptor Subunit α-5 (NACHRA5) is a member of the ligand-gated ion channel family. Neuronal AChR is composed of two different type of subunits: α and non-α. When NACHRA5 binds to acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits, leading to the opening of an ion-conducting channel across the plasma membrane. Genetic variations in NACHRA5 have been related to susceptibility to smoking-related behavioral traits and lung cancer, contributing to the smoking quantitative trait locus 3.

Sequence: Arg23-Thr254

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 27.6 kDa

Observed MW: 35-40 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only