Cyclin H Monoclonal Antibody

Catalogue Numbers: MB66662-50, MB66662-100

Sizes: 50µl, 100µl

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P51946

Host: Mouse

Reactivity: Human, Mouse

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: Cyclin H belongs to a conserved cyclin family that plays a critical role in the regulation of cell cycle dependent kinases (CDKs) necessary for cell cycle progression. In general, the activity of CDKs requires the binding of appropriate cyclins as well as phosphorylation driven by Cdk-activating kinase (CAK). Cyclin H is part of the CAK complex that includes the kinase CDK7, and an assembly factor p36/Mat1, which enhances binding between cyclin H and CDK7 and increases activity. CAK regulates progression through the cell cycle by activating cdc2, CDK2, and CDK4 kinases through phosphorylation of a critical threonine residue in the T-loop of the CDK-cyclin complexes. The CAK complex can exist either in its free form or in association with transcription factor IIH (TFIIH) which can affect its substrate specificity. When bound to TFIIH, CAK preferentially phosphorylates the carboxy-terminal domain of RNA polymerase II, providing a link between cell cycle control, transcriptional regulation, and DNA repair.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Cyclin H protein.

Extra Notes: Western blot analysis of Cyclin H expression in Jurkat (A), K562 (B), Hela (C), NIH3T3 (D) whole cell lysates.

Bioworld Molecular Weight: ~ 36 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Cyclin-H; MO15-associated protein; p34; p37

Immunogen: Purified recombinant human Cyclin H protein fragments expressed in E.coli.

Conjugate: Unconjugated

Modification: Unmodified