BUBR1 Polyclonal Antibody

Product: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16811-50, BZ16811-100

Swiss-Prot: O60566

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P, IP, FC

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20 FC: 1/50-1/100

Background: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Calculated MW: 120 kDa; Observed MW: 120 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of BubR1 in HepG2 lysates using BUBR1 antibody.

Alternative Name: BUB1B; BUBR1; MAD3L; SSK1; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L; Protein SSK1

Immunogen: A synthesized peptide derived from human BubR1

Conjugate: Unconjugated

Modification: Unmodified