Target Antigen

Nidogen/Enactin

Quantity

0.1ml 

Clone

JF3

Host

Rat

Immunogen

Recombinant fragment corresponding to the G1/Link region of mouse nidogen

Myeloma/fusion partners

Fisher rat plus YB2/0

Species Reactivity

Mouse, Human

Purification

Protein G

Format

Affinity purified antibody from culture supernatant in 0.5 M acetic acid neutralized with 2 M Trizma + 0.1% sodium azide

Applications

WB, ELISA, Blocking

Dilutions

Optimal antibody dilution should be determined by titration, however as a guideline try;

BL: Use at an assay dependent dilution. Blocks binding of nidogen to laminin. 

WB: Use at an assay dependent dilution. Predicted molecular weight: 136 kDa

References

Mapping of nidogen binding sites for collagen type IV, heparan sulfate proteoglycan, and zinc. Reinhardt, D. et al. ( J Biol Chem,1993) PUBMED

Research areas

Cell Biology; Cytoskeleton / ECM; Neuroscience;

Also known as

Ent antibody;Entactin 1 antibody;NID 1 antibody;NID antibody;Nid1 antibody;Nidogen (enactin)antibody;Nidogen 1 antibody;Nidogen antibody

Primary Description

Rat Anti-Nidogen/Enactin [JF3]

Catalogue Number

IQ312

Database links

Entrez Gene: 4811 Human

Entrez Gene: 18073 Mouse

Omim: 131390 Human

SwissProt: P14543 Human

SwissProt: P10493 Mouse

Unigene: 356624 Human

Unigene: 4691 Mouse

Specificity

Recognises native and denatured  nidogen 1, and blocks binding of nidogen to laminin. Epitope Located in the G1/Link region of Nidogen 1 

Concentration

1mg/ml

Type

Isotype

IgG2a

Nature

Purity