Mouse SIRT1 (Sirtuin 1) ELISA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-EL-M0350-24, E-EL-M0350-48, E-EL-M0350-96

Citations, Manuals and MSDS Available upon request.

Abbreviation: SIRT1

UNIProt ID: Q923E4

Target Synonyms: SIR2L1; SIR2alpha; sir2-like 1; sirtuin 1; Silent Mating Type Information Regulation 2 Homolog 1;

Research Areas: Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Metabolism, Microbiology, Tags and Cell Markers

Target Species: Mouse

Application: ELISA

Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse SIRT1 concentrations in serum, plasma and other biological fluids.

Tested Sample Types: Serum, Plasma and other biological fluids

Type: Sandwich

Assay Time: 3.5h

Detection Type: Colormetric

Sensitivity: 0.1 ng/mL

Detection Range: 0.16-10 ng/mL

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SIRT1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SIRT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SIRT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SIRT1. You can calculate the concentration of Mouse SIRT1 in the samples by comparing the OD of the samples to the standard curve.

Cross Reactivity: This kit recognizes Mouse SIRT1 in samples. No significant cross-reactivity or interference between Mouse SIRT1 and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 12 months

Shipping: ice bag